Team:Queens-Canada/12 July 2010

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July 12, 2010

Liquid Culture

Thai

pBSII

PCR

Hao and Basia

pOdr-10, pOdr-1, pSrb-6, pSra-10, pOsm-10, pCeh-12, pFlp-1, pStr-1

Program run using HIFI with an extension time of 140 s and annealing temperature of 55 °C.

Gel Electrophoresis

Hao

pOdr-10, pOdr-1, pSrb-6, pSra-10, pOsm-10, pCeh-12, pFlp-1, pStr-1

Used 0.7 g agarose in 100 mL TBE. Gel run for 55 mins at 100 V.

Microinjection

Hao Practiced injections with water.

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