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From 2010.igem.org

For one liter of 0.5 [http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium MS]:

• 2.165 g MS salt
• 15 g sucrose (1.5%)
• 12 g agar (1.2%)
• 1 N NaOH or KOH

Procedure

Use container that is twice as large as solution volume (ex. for 1 L of media, use a 2 L flask). Add MS salt and sucrose to 900mL distilled water. Adjust pH to 5.7 using 1 N NaOH or KOH solutions. Finally, add agar. There is no need to dissolve the agar prior to autoclaving.

Autoclave Notes

Cover flask/container with double layer aluminum foil. Place in AutoClave and select P6-Liquid-Long Cycle. Total time for AutoClave is 60 min.