Team:Harvard/parts
From 2010.igem.org
parts
name | biobrick name | original name | description | resistance (bacteria) | resistance (plants) | sequence |
V7 | BBa_K382000 | pORE Open Series Vector with BioBrick Sites (kan/pat) | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | V7 |
V8 | BBa_K382001 | pORE Open Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | V8 |
V9 | BBa_K382002 | pORE Expression Series Vector with BioBrick Sites (kan/pat) | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | V9 |
V10 | BBa_K382003 | pORE Expression Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | V10 |
V11 | BBa_K382004 | pORE Reporter Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | V11 |
V12 | BBa_K382005 | pORE Reporter Series Vector with BioBrick Sites (kan/nptII) | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | V12 |
C1 | BBa_K382010 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown | chloramphenicol | - | amiRNA LUT2 |
C2 | BBa_K382011 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | amiRNA BETA-OHASE 1 |
C3 | BBa_K382012 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | amiRNA LYC |
A2 | BBa_K382013 | amiRNA LTP | LTP knockdown construct | chloramphenicol | - | amiRNA LTP |
A3 | BBa_K382014 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | - | ihpRNA Bet1 |
A4 | BBa_K382015 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | ihpRNA LTP |
A4 | BBa_K382018 | PDK intron hairpin | Intron hairpin construct | ampicillin | - | |
A5 | BBa_K382019 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | ihpRNA GER |
F1 | BBa_K382017 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | link |
F2 | BBa_K382018 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | link |
F3 | BBa_K382019 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on 5' end. | chloramphenicol | - | link |
F4 | BBa_K382020 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | link |
F5 | BBa_K382021 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | link |
F6 | BBa_K382022 | Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link |
F7 | BBa_K382023 | Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | link |
F8 | BBa_K382024 | Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
F9 | BBa_K382025 | Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | link |
GF3 | BBa_K382035 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | link |
GF4 | BBa_K38202 | RXRLc | Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide | chloramphenicol | - | link |
GF5 | BBa_K382030 | RXRHm | Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide. | chloramphenicol | - | link |
GF6 | BBa_K382034 | VP16 | Part of the Retinoic Acid Receptor and VP16 activation domain fusion protein. VP16 recruits transcription factors, and when colocalized with the Gal4 Upstream Activating Sequence initiates transcription downstream of the 5xGal435s promoter. | chloramphenicol | - | link |
GF7 | BBa_K382035 | Ectysone Receptor | Part of the fusion protein of Ectysone receptor and Gal4 DNA binding domain. Ectysone receptor binds RXR-VP16 in presence of Methoxyfenozide | chloramphenicol | - | link |