Team:Wisconsin-Madison/results
From 2010.igem.org
Encapsulation
Colonic Acid Quantification
Encapsulation: Cell Survivability in Acidic Conditions
Timed Lysis: Inducible/Repressible Promoter
gadAp: A pH Sensitive Promoter
Based on available literature (cite), we expected an increase in expression from gadAp (BBa_K318512) under both acidic pH conditions (from pH 5 to 3) and in the stationary phase (after an OD of 1 or 2). In order to test this, we combined the gadA promoter with a RFP gene (BBa_K318513). This part
pH Induction
Stationary Phase Induction