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Team:Berkeley/Results
From 2010.igem.org
- Home
- Project
- Parts
- Self-Lysis
- Vesicle-Buster
- Payload
- [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Berkeley Parts Submitted]
- Results
- Judging
- Clotho
- Human Practices
- Team Resources
- Who We Are
- Notebooks:
- [http://www.openwetware.org/wiki/Berk2010-Daniela Daniela's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Christoph Christoph's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Amy Amy's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Tahoura Tahoura's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Conor Conor's Notebook]
Results of Delivery of GFP payload with Payload Delivery Device
Experimental Set Up
In general to assay payload delivery, we first
- Feed the payload bacteria to the choanos and induce self-lysis, not necessarily at the same time.
- Next, we assay for successful delivery events.
SInce the payload we used in our assays was GFP, we assayed for delivery events using fluorescent microscopy.
Challenges
The major challenge we faced with assaying for payload delivery was determining the timing of when to induce self-lysis and when to look at the choanos under the microscope.
