Week of 10/4

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October 5th

Digests:

-R1062+RFP (SP)
-R0061+RFP (SP)

Ligations:

-R1062+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)

October 6th

Colony PCR:

1. R1062+RFP + J23100+LuxR
2. R1062+RFP + J23108+LuxR
3. R1062+RFP + J23116+LuxR
4. R0063+RFP + J23100+LuxR
5. R0063+RFP + J23108+LuxR
6. R0063+RFP + J23116+LuxR

They were then run on a gel after being PCRd.

Gel Order:

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