Team:Stockholm/17 October 2010
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Contents |
Mimmi
clone non-CPP operon
colony PCR
mix | x12 | primers | samples | |||
---|---|---|---|---|---|---|
mastermix | 24.5 | pEX_VF | pEX.SOD.RBS.yCCS 1-4 | |||
DNA | 0.5 | pEX_VR | pEX.SOD.his.RBS.yCCS 1-4 | |||
tot | 25µl | pEX.his.SOD.RBS.yCCS 1-4 | ||||
positive control (pSB1C3.LMWP.SOD.his) |
Gel
well | sample | well | sample |
---|---|---|---|
1 | ladder | 12 | ladder |
2 | pEX.S_yC 1 | 13 | pEX.hS_yC 1 |
3 | pEX.S_yC 2 | 14 | pEX.hS_yC 2 |
4 | pEX.S_yC 3 | 15 | pEX.hS_yC 3 |
5 | pEX.S_yC 4 | 16 | pEX.hS_yC 4 |
6 | pEX.Sh_yC 1 | ||
7 | pC.Sh_yC 2 | ||
8 | pC.Sh_yC 3 | ||
9 | pC.Sh_yC 4 |
new colony PCR
mix | x7 | primers | samples | |||
---|---|---|---|---|---|---|
mastermix | 24.5 | pEX_VF | pEX.SOD.his.RBS.yCCS 1-7 | |||
DNA | 0.5 | pEX_VR | ||||
tot | 25µl |
ON cultures
- pEX.SOD.RBS.yCCS 4
- pEX.his.SOD.RBS.yCCS 3
- pEX.SOD
- pEX.yCCS
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