"
User:AndreasConstantinou/29 June 2010
From 2010.igem.org
Revision as of 16:00, 29 June 2010 by AndreasConstantinou (Talk | contribs)
Expression of SOD and yCCS from pEX expression vector
Two cell cultures of DH5alpha cells respectively transformed with pEX vectors carrying superoxidase dismutase (SOD) and yeast copper chaperone (yCCS) were previously prepared and received from Nina and Johan.
Procedures
- An ON starter culture was set by inoculating 5 ml LB + 100 ug/ml Amp with 5 ul of each culture.
- 37°C, 250 rpm.
Continued 30/6
