Team:Yale/Our Project/Notebook/Week 2

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iGEM Yale

lab notebook: week 2 (6/14-6/20)

  • Monday 6/14--Primer design, redo of Friday's growth assay
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  • Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived
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  • Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions
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  • Thursday 6/17--more minimal media work and meeting, started BL21 culture
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  • Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges. June the 19th--in which it is revealed that there was a transformation-fail Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge
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  • Sunday 6/20--in evening inoculate 5 mL liquid cultures
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