Material and Methods
Our material for B-IO Guide Software Program was the all background
information of parts stated in distributed 2010 iGEM plates to whole
wetlab teams. Data of a part having specific part ID has been parsed
by writing a parsing code. Then, other data which needs to be standardized
according to biological importance which has been get out from the
Registry of Parts Page manually. These standardized data are;
- working conditions of biobricks involved within the parts
- properties of inputs and outputs
We named as "Inputs" because an effector which
may be chemical(IPTG, galactose etc.) and physical (UV irradiation,
temperature etc) external inputs or may be proteins synthesized
from a biobrick coding sequence can affect promoters on the parts.
The effects of these mentioned inputs have been determined as inducer
(a molecule that starts gene expression), repressor (blocker of
attachment of RNA polymerase to promoter), activator (increasing
the rate of transcription) and inhibitor (decreasing the rate of
transcription). Moreover, the promoters may have additional property
at which they may become constitutively ON without seen any effect.
On the other hand, for output section, we add new approach which
is "working condition" parameter because the expressed proteins
from biobrick coding sequences may come from different organisms
to run mostly in E.coli. However, in some cases these proteins may
not work well as expected unless suitable and optimum conditions
are provided in the host. Thus, we have thought that natural(in
vivo) or experimental(in vitro) working conditions of the synthesized
proteins from the certain biobricks or constructs should be stated
during submitting to Parts Registry. Nevertheless, our 2010 iGEM
Software Program version 1.0 mostly does not have any experimental
data since the data had not been added for pages of the parts regularly
and true. Therefore, if our advised Parts Registry interfaces are
used in iGEM in future and our standards are used, then we will
be able to add such an information to the database of the program
for better performance.