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From 2010.igem.org

Production Phase

Suggested Articles

http://www.ncbi.nlm.nih.gov/pubmed/17536514

http://www.ncbi.nlm.nih.gov/pubmed/17571257

http://www.ncbi.nlm.nih.gov/pubmed/19892171

http://www.ncbi.nlm.nih.gov/pubmed/20201800

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Hassan: For production phase, there are several cases that we need to take care of. One of interesting subjects is "inclusion bodies". As Johan and Nina said, it is better to study about over expression.

Secretion

http://www.ncbi.nlm.nih.gov/pubmed/15763404

http://www.ncbi.nlm.nih.gov/pubmed/19597765

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PPI

"It has become dangerously acceptable to conclude that proteins interact directly provided that (i) the interaction is biologically plausible; (ii) the proteins are co-expressed; and (iii) an interaction is demonstrated by glutathione-S-transferase (GST)-pulldowns and/or co-immunoprecipitations (co-IPs)."

"The most obvious caveat with co-IP data is that a positive result does not imply a direct interaction between two proteins – binding could be mediated by other partners."

"Protein interactions: is seeing believing? J. P. Mackay et al. 2007"