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Contents 1 Overall project 2 Project Details 2.1 signalling Lac Repressor Signaling 2.2 Volatile Cell Signalling 2.3 Non-Volatile Cell Signalling 2.4 AI-2 Purification 2.5 Chitin Synthase 2.6 Chitinase Knockout (may not be necessary) 2.7 Holin 3 Parts

Overall project

Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)

in chitinase-KO strain e coli

Project Details

signalling Lac Repressor Signaling

http://openwetware.org/wiki/IPTG

Volatile Cell Signalling

phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA

article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411

Non-Volatile Cell Signalling

registry: http://partsregistry.org/Cell-cell_signalling

quorum sensing: LuxR, LuxS, LuxpR

AI-2 Purification

http://www.pnas.org/content/suppl/2003/06/25/1537100100.DC1/7100SuppText.html

Chitin Synthase

chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311

UDP-GlcNAc -> UDP + Chitin

Chitinase Knockout (may not be necessary)

summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts

chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837

chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300

Holin

registry: http://partsregistry.org/Cell_death

Parts

RBS

BBa_B0029	TCTAGAGTTCACACAGGAAACCTACTAGATG	-	0.764
BBa_B0030	TCTAGAGATTAAAGAGGAGAAATACTAGATG	0.6	-
BBa_B0031	TCTAGAGTCACACAGGAAACCTACTAGATG	0.07	-
BBa_B0032	TCTAGAGTCACACAGGAAAGTACTAGATG	0.3	0.376
BBa_B0033	TCTAGAGTCACACAGGACTACTAGATG	0.01	0.002
BBa_B0034	TCTAGAGAAAGAGGAGAAATACTAGATG	1	1
BBa_B0035	TCTAGAGATTAAAGAGGAGAATACTAGATG	-	1.124
BBa_B0064	TCTAGAGAAAGAGGGGAAATACTAGATG	0.35	-