Team:Harvard/parts
From 2010.igem.org
parts and primers
Name | Original Name | Description | Resistance | Resistance (Plants) | Sequence |
V1 | pORE Open Series 1 (O1) | Agrobacterium vector open series | Kanamycin | pat | pORE_O1 |
V2 | pORE Open Series 2 (O2) | Agrobacterium vector open series | Kanamycin | nptII | pORE_O2 |
V3 | pORE Expression Series 3 (E3) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | pat | pORE_E3 |
V4 | pORE Expression Series 4 (E4) | Agrobacterium vector with ENTCUP2 promoter | Kanamycin | nptII | pORE_E4 |
V5 | pORE Reporter Series 1 (R1) | Agrobacterium vector with gusA reporter | Kanamycin | nptII | pORE_R1 |
V6 | pORE Reporter Series 3 (R3) | Agrobacterium vector with smgfp reporter | Kanamycin | nptII | pORE_R3 |
V7 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | pat | V7:pORE_O1 biobricked | |
V8 | Agrobacterium vector open series with biobrick cloning site | Kanamycin | nptII | V8:pORE_O2 biobricked | |
V9 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | pat | V9:pORE_E3 biobricked | |
V10 | Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site | Kanamycin | nptII | V10:pORE_E4 biobricked | |
V11 | Agrobacterium vector with gusA reporter and biobrick cloning site | Kanamycin | nptII | V11:pORE_R1 biobricked | |
V12 | Agrobacterium vector with smgfp reporter and biobrick cloning site | Kanamycin | nptII | V12:pORE_R3 biobricked | |
A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | - | amirnaGFP |
A2 | amiRNA LTP | LTP knockdown construct | chloramphenicol | - | amiRNA LTP |
A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | - | ihpRNA Bet1 |
A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | - | ihpRNA LTP |
A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | - | ihpRNA GER |
F1 | pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. | chloramphenicol | - | |
F2 | NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. | chloramphenicol | - | |
F3 | NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on end. | chloramphenicol | - | |
F4 | Brazzein | Sweet-tasting protein. 54 amino acid sequence. | chloramphenicol | - | |
F5 | Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence | chloramphenicol | - | |
F6 | Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | |
F7 | Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression | chloramphenicol | - | |
F8 | Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | |
F9 | Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. | chloramphenicol | - | |
C1 | amiRNA LUT2 | amiRNA construct for lycopene epsilon cyclase (LUT2)knockdown | chloramphenicol | - | |
C2 | amiRNA BETA-OHASE 1 | amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown | chloramphenicol | - | |
C3 | amiRNA LYC | amiRNA construct for lycopene beta cyclase (LYC) knockdown | chloramphenicol | - | |
GF1 | Barnase | Fatal gene for the genetic fence | chloramphenicol | - | |
GF2 | Barstar | Protein inhibitor of Barnase | chloramphenicol | - | |
GF3 | LacIN | Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter | chloramphenicol | - | |
GF4 | RXRLc-VP16 | Fusion protein of Locust Retinoic Acid Receptor and VP16 activation domain - binds EcR-Gal4 in presence of Methoxyfenozide | chloramphenicol | - | |
GF5 | EcR-Gal4 | Fusion protein of Ecdysone receptor and Gal4 DNA binding domain - binds RXRLc-VP16 and initiates transcription downstream of Gal Upstream Activating Sequence in presence of Methoxyfenozide | chloramphenicol | - |