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Team:EPF Lausanne/Project droso
From 2010.igem.org
Drosophila
In parallel, we did experiments to assess if Asaia was persistant in Drosophila melanogaster . In fact, the fly is a much easier organism to grow and work on, in opposition to mosquitoes which need to feed on animals to reproduce. Our experiment was set in 3 steps:
- infection of the flies with GFP-Asaia*, and 3 other bacteria as controls
- retrieval of the flies after 3h (control of infection), 24h, 48h
- counting of the number of colonies for all time points and the 4 differents strains
The goal was to observe the persistance of Asaia in the Drosophila gut to see if it can be used as a model organism to study Asaia.
The results were negative for a the experience and we repeteated it 4 times.
graphe des drosophiles
