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Team:Stockholm/23 October 2010
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Andreas
IgG protease activity assay
Finished the repeated experiment and read the wavelength at 450 nm.
Blank: 80 μl PBS + 100 μl SureBlue™ peroxidase substrate + 100 μl 1 M HCl.
| Sample loading | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ab dil. | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| 1:500 | A | Ind. IgGp A | Ind. IgGp B | Ind. SOD A | Ind. SOD B | Unind. IgGp A | Unind. IgGp B | ||||||
| 1:250 | B | Ind. IgGp A | Ind. IgGp B | Ind. SOD A | Ind. SOD B | Unind. IgGp A | Unind. IgGp B | ||||||
| – | C | PBS 1:500 | PBS 1:250 | Blank | – | – | – | – | – | – | – | – | – |
Results
| A450 measurements | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| A | 0.073 | 0.075 | 0.087 | 0.087 | 0.091 | 0.094 | 0.089 | 0.090 | 0.131 | 0.132 | 0.109 | 0.108 |
| B | 0.116 | 0.126 | 0.116 | 0.117 | 0.156 | 0.158 | 0.145 | 0.150 | 0.181 | 0.187 | 0.125 | 0.124 |
| C | 0.003 | 0.011 | 0.000 | – | – | – | – | – | – | – | – | – |
