Team:TU Delft/23 June 2010 content

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LAB WORK

Ligations were performed using the digested BioBricks obtained in the early morning. The ligation protocol was in accordance with the following:

-	uL
I10341	3
J611XX	4
pSB3C5	1.5
Ligation Buffer	3
T4 ligase	1 unit
H20	15

We adhered to the 3:1 insert:plasmid ratio when determining the volumes of DNA added. The negative control for the ligation consisted of J61100 + pSB3C5 and the negative control for the transformation was pure H2O. We're hoping for some nice transformants we can finally characterize tomorrow!