Team:Newcastle/21 June 2010

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Monday

Wet Lab techniques practice

Aims: The aim of today's Lab training session was to practice aseptic technique and wet lab techniques such as streak plating and broth culture.

Equipement list:

For today's session we needed

• Agar plates
• Pipettes
• Wire loops
• E.coli (from a colony)
• LB broth
• Bunsen burner
• Conical flasks
• Orbital shaker

Tips:

• Use Aseptic technique: Treat everything as a pathogen!
• Work around the bunsen burner.
• Use a control.
• Clean the bench at the end of the day!
• Heat the wire loop from the middle to the tip.
• Keep plates upside down.
• Keep lids off for as short a time as possible.
• Loosen all the tops off the vessels before you start.
• Flame tops of bottles lightly.

Streak plating

Star plate streaking student, Deena, shows off how it is to be done!

Set up culture for minipreps

5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.