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Team:Aberdeen Scotland/Curve Fitting
From 2010.igem.org
University of Aberdeen - ayeSwitch
iGEM 2010
Curve Fitting to find the Hill Coefficient for the GFP/Bbox-stem Association (n2)
Based on a graph in a paper by Witherell et al.[14] which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n1. Our two MS2 stem loops (see Fig 1 in Equations) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).
Figure 5. A. Graph from paper by Witherell et al.[14] showing the binding curves of the MS2 stem loop. The filled squares are the 8-16 construct which closely resembles the binding curves of our MS2 stems. B. The binding curve for the 8-16 construct was reproduced in MATLAB and the Hill function for activators equation fitted to it (red line).
