Curve Fitting to find the Hill Coefficient for the GFP/Bbox-stem Association (n₂)

Based on a graph in a paper by Witherell et al.^[14] which showed the binding curves of the MS2 stem loop we could calculate more accurately the value for n₁. Our two MS2 stem loops (see Fig 1 in Equations) are 19 nucleotides apart, so our binding curve will most closely resemble that of the 8-16 construct, shown in figure 5A (filled squares).