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From 2010.igem.org

Contents 1 2010/10/14 1.1 1:Electrophoresis 1.2 2：DNA extraction 1.3 3：DNA Ligation 1.4 4：DNA Digestion 1.5 5:PCR 1.6 6：DNA extraction from gels 1.7 7：DNA Digestion 1.8 8:PCR 1.9 9：DNA extraction from gels 1.10 10:Electrophoresis 1.11 11：DNA extraction from gels

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

• 1-2-7
• 1-2
• 10-2-7
• 9-7
• 4-5

<Protocol>
See Protocol 8

<Result>

2：DNA extraction

＜Member＞
nito

＜Sample＞

• 1-2-7
• 10-2-7
• 9-7

＜Protocol＞
See Protocol 4

3：DNA Ligation

＜Member＞
nito

＜Sample＞

• 4-5
• 1-2-3
• 10-2-3
• 10-2-9
• 7-8-10

(after digestion)

＜Protocol＞
See Protocol 10

4：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

• plasmid

・Digest enzyme

• XbaⅠ
• SpeⅠ

＜Protocol＞
See Prptpcpl 9

5:PCR

<Member>
nito

<Sample>

• vector
• 4-5
• 1-2-3
• 10-2-3
• 7-8-10
• 10-2-9

<Protocol>
See Protocol 1

6：DNA extraction from gels

＜Member＞
nito

＜Sample＞

• 9-7

(After electrophoresis)

＜Protocol＞
SeeProtocol 4

7：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions

• 1-2
• 1-2-7
• 10-2-7

・Digest enzyme

• AvrⅡ
• NheⅠ
• XbaⅠ
• SpeⅠ

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
nito

<Sample>

• 9
• 9-7

<Protocol>
See Protocol 1

9：DNA extraction from gels

＜Member＞
nito

＜Sample＞

• 9-7

(After electrophoresis)

＜Protocol＞
SeeProtocol 4

10:Electrophoresis

<Member>
nito

<Sample>

• vector
• 9-7
• 4-5

<Protocol>
See Protocol 8

<Result>

11：DNA extraction from gels

＜Member＞
nito

＜Sample＞

• 9-7
• 4-5

(After electrophoresis)

＜Protocol＞
SeeProtocol 4