From 2010.igem.org
Microscopy
Details on microscope
Figure 1: Picture of the automatized microscope.
A microscope with motorized x, y and z control, a motorized shutter and a 60× lens is used with appropriate fluorescence filters for the fluorescence signals. Light-emitting diode arrays are installed as light sources for red light (660 nm) and far-red light (748 nm) pulses.
Details on the Flow Channel
 Figure 2: The mold for the channel. Has to be cleaned after casting. |  Figure 3: The casted raw channel with a cover slip and the razor plate needed for preparing the channel. |  Figure 4: The assembled channel, yet not bonded. |
 Figure 5: Bonding the channel. |  Figure 6: The ready to use channel under the microscope. |
Details on the red light/ far-red light diodes and the filters
 Figure 7: The approximated spectral energy irradiances of the red light (red curve) and far-red light (blue curve) diodes. |  Figure 8: The amplifications of the red-light (red curve) and far-red light (blue curve) filters. |  Figure 9: The photoconversion cross-sections of PhyB in the Pr (red curve) and Pfr (blue curve) states. |
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