Team:ETHZ Basel/InformationProcessing/Microscope

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Microscopy

Details on microscope

Figure 1: Picture of the automatized microscope.
A microscope with motorized x, y and z control, a motorized shutter and a 60× lens is used with appropriate fluorescence filters for the fluorescence signals. Light-emitting diode arrays are installed as light sources for red light (660 nm) and far-red light (748 nm) pulses.


Details on the Flow Channel

Figure 2: The mold for the channel. Has to be cleaned after casting.
Figure 3: The casted raw channel with a cover slip and the razor plate needed for preparing the channel.
Figure 4: The assembled channel, yet not bonded.
Figure 5: Bonding the channel.
Figure 6: The ready to use channel under the microscope.

Details on the red light/ far-red light diodes and the filters

Figure 7: The approximated spectral energy irradiances of the red light (red curve) and.
Figure 8: The casted raw channel with a cover slip and the razor plate needed for preparing the channel.
Figure 9: The assembled channel, yet not bonded.