Figure 1. Absorbance spectrum of Synechocystis sp. PCC 6803 and the excitation and emission wavelengths of CFP, GFP, YFP, and RFP. Absorbance spectrum (black line) adaptated from Wilde, et al. (1995). Fluorescent proteins emission and excitation wavelengths shown as a single bar due to small relative distance between wavelengths, bars are color coded to the proteins.
Figure 2. Construction of M11400 from pIGA. (1) Mutagenesis of EcoRI site. (2) Replacement of GFP cassette with BioBrick prefix and suffix. (3) Mutagenesis of EcoRI and PstI sites. (4) Insertion of NheI digested region from BioBrick Basic Vector.
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