O/N Culture

From 2010.igem.org

Revision as of 19:24, 16 October 2010 by Bzhang89 (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

REMEMBER TO BE STERILE


  • Take 5mL LB and put in a round bottom tube
  • If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube 
  *MAKE SURE YOU ADD THE RIGHT ONE!
  • Take a tip on a p200 and lightly touch a colony
  • Eject the tip into the culture and put the cap on
  • For a miniprep, grow O/N for 12-16 hours NOT LONGER

[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware

Back to Protocol

Retrieved from "http://2010.igem.org/O/N_Culture"