Team:Yale/LabNotebook/Week2

From 2010.igem.org

Revision as of 17:15, 20 June 2010 by Litagaki (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Monday 6/14--Primer design, redo of Friday's growth assay

Tuesday 6/15--more primer design & plasmid synthesis planning (see posted plan on google group), spotted cell soln's from Monday growth assay to see if bacteria survived

Wednesday 6/16--checked on spotted cell survival assay, collected MOPS minimal media materials, started making component solutions

Thursday 6/17--more minimal media work and meeting, started BL21 culture

Friday 6/18--plasmid pSB74 arrived (!), already in some unspecified E coli, so plated them out. Transformed promoters (constitutive & IPTG inducible), terminator, and repressor (for inducible promoter) into BL21. Also ran BL21 copper growth assays for wide and narrow concentrations ranges.

June the 19th--in which it is revealed that there was a transformation-fail

Redid transformation, this time into LE392, set aside pSB74 containing cells in fridge

Sunday 6/20--in evening inoculate 5 mL liquid cultures