Team:Stockholm/14 October 2010

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Revision as of 17:16, 14 October 2010 by AndreasConstantinou (Talk | contribs)


Contents

Andreas

Growth curve assay

New attempt to measure growth curves for CPPs.

Induction

  • 150 ml side-arm E-flasks
  • 15 ml LB
  • 150 μl ON culture (1:100)
    • BL21
    • ON cultures set by Mimmi 13/10
  • 37 °C, 220 rpm

OD590 measurements

  0 min 30 min 60 min 90 min 120 min 150 min 180 min 210 min 240 min 270 min
pEX.SOD⋅His 0.03 0.04 0.10 0.23 0.45 0.78 1.06 1.36 1.56 1.67
pEX.nTra10⋅SOD⋅His 0.03 0.04 0.09 0.19 0.36 0.61 0.94 1.18 1.42 1.56
pEX.nTAT⋅SOD⋅His 0.03 0.05 0.10 0.23 0.49 0.78 1.06 1.35 1.55 1.66
pEX.nLMWP⋅SOD⋅His 0.04 0.05 0.11 0.25 0.52 0.88 1.14 1.40 1.60 1.69

Experiment was canceled after 270 min as I was made aware that the spectrophotometer only makes accurate measurements up to ≈OD590 = 0.5.

ON cultures

New ON cultures set (including two for Mimmi)

  • 5 ml LB + Amp 100; 37 °C, 225 rpm
    • BL21, pEX.SOD⋅His
    • BL21, pEX.nTra10⋅SOD⋅His
    • BL21, pEX.nTAT⋅SOD⋅His
    • BL21, pEX.nLMWP⋅SOD⋅His
    • (BL21, pEX.SOD)
    • (BL21, pEX.yCCS)