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From 2010.igem.org
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- 16:15, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols/Gel electrophoresis
- 16:15, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols/PCR purification from Agarose Gel
- 16:15, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols/Restriction biobrick parts (top)
- 16:14, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols/miniprep (top)
- 16:14, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols/Transformation of competent cells
- 16:13, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols/makinglb
- 14:56, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:56, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/ Enzymatic activity with Victor machine (New page: ==Scientific Background== The Wallac 1420 VICTOR2 is a multilabel, multitask plate reader designed to support the future demands of industrial and academic laboratories for multiple assay ...)
- 14:55, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/Ligand Treatment (New page: ==Scientific Background== Ligand treatment is a procedure when we add the appropriate ligand to the nuclear receptor (NR, transcription factor). The NR got into the cells through a previo...)
- 14:54, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:54, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/ Transfection (New page: Transfection protocol with PEI nuclear receptor construct (for COS1 cells) ==Scientific Background== The following protocol is used to succesfully introduce foreign plasmid DNA into plur...)
- 14:53, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/ Media PEI Preparation (New page: Preparing medias for eukaryotic cell culturing, preparing PEI solution for transfection ==Scientific Background== I. The Media Eukaryotic cells, derived from multicellular animal eukaryo...)
- 14:51, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:49, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:48, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:47, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/Cell Passaging (New page: ==Scientific Background== Medium reconstitution: You have to reconstitute a new bottle of medium when you don't have enough medium for the subsequent processes (cell culturing, Transfecti...)
- 14:46, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/Gel electrophoresis (New page: Gel Electrophoresis: ==Scientific Background== Gel electrophoresis is a technique used for the separation of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein molecules usin...)
- 14:45, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/Restriction biobrick parts (New page: ==Scientific Background== BioBrick standard biological parts are flanked by well characterized upstream and downstream sequences which are technically not considered part of the BioBric...)
- 14:44, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:44, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/PCR purification from Agarose Gel (New page: DNA Fragments Purification from Agarose Gel ==Scientific Background== ==Overview== ==Materials== Centrifuge Vortex Incubator Pipet (100-1000µl and 2-20µl) Tips (blue and yellow) ...)
- 14:41, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/Transformation of competent cells (New page: ==Scientific Background== Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are call...)
- 14:41, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:39, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/makinglb (New page: ==Scientific Background== Giuseppe Bertani published the original formula of LB, which is used for the cultivation of E. Coli. It provides the main essential nutrition, containing peptides...)
- 14:38, 4 October 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 14:37, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/protocols/miniprep (New page: ==Scientific Background== This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1–5 ml overnight cultures of E. coli in LB (Luria-Bertani) medium. =...)
- 14:36, 4 October 2010 (diff | hist) N Team:Debrecen-Hungary/miniprep (New page: ==Scientific Background== This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1–5 ml overnight cultures of E. coli in LB (Luria-Bertani) medium. =...) (top)
- 17:04, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 17:03, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 17:02, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 16:58, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 16:57, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 16:53, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 16:52, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 16:48, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/protocols
- 16:43, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 14:56, 28 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 21:19, 27 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 21:19, 27 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 04:24, 27 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 19:15, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 19:09, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 19:08, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 19:06, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 19:05, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 19:04, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 18:54, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 18:52, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 18:51, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 18:50, 26 September 2010 (diff | hist) Team:Debrecen-Hungary/minimals
- 18:05, 25 June 2010 (diff | hist) Team:Debrecen-Hungary/Modeling
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