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User contributions
From 2010.igem.org
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- 13:12, 22 October 2010 (diff | hist) Team:Panama/23 September 2010
- 13:11, 22 October 2010 (diff | hist) Team:Panama/22 September 2010
- 13:09, 22 October 2010 (diff | hist) Team:Panama/16 September 2010
- 13:08, 22 October 2010 (diff | hist) Team:Panama/15 September 2010 (→July 23)
- 13:07, 22 October 2010 (diff | hist) Team:Panama/15 September 2010
- 13:06, 22 October 2010 (diff | hist) Team:Panama/2 August 2010 (→August 02)
- 13:05, 22 October 2010 (diff | hist) Team:Panama/5 September 20103 (→September 05)
- 13:03, 22 October 2010 (diff | hist) Team:Panama/5 September 20103
- 13:01, 22 October 2010 (diff | hist) Team:Panama/13 August 2010
- 12:59, 22 October 2010 (diff | hist) Team:Panama/28 September 2010
- 12:58, 22 October 2010 (diff | hist) Team:Panama/4 October 2010
- 12:55, 22 October 2010 (diff | hist) Team:Panama/4 October 2010
- 23:53, 21 October 2010 (diff | hist) Team:Panama/12 October 2010
- 23:52, 21 October 2010 (diff | hist) Team:Panama/2 August 2010 (→August 02)
- 23:52, 21 October 2010 (diff | hist) Team:Panama/19 October 2010
- 23:51, 21 October 2010 (diff | hist) N Team:Panama/19 October 2010 (New page: PCR of Rh1ab *Table Electrophoresis: Miniprep samples 1-5 in 0.8% agarose and 1x TBE *Photo We observe bands around 5000bp. It could be the linear plasmid DNA Sample 4 was the only on...)
- 23:45, 21 October 2010 (diff | hist) N Team:Panama/18 October 2010 (New page: Inoculation We inoculated 5 colonies in liquid LB + amp medium -3 ml medium + 1 colony We incubated them overnight at 37C with shaking Prepare other aliquot of primer for the amplifi...)
- 23:31, 21 October 2010 (diff | hist) N Team:Panama/16 October 2010 (New page: PCR Rh1ab *Photo PCR of the sample from well 2 (15 oct) *Photo)
- 23:29, 21 October 2010 (diff | hist) N Team:Panama/14 October 2010 (New page: Prepared Ampicillin stock (50 mg/ml) *Photo We did a miniprep on the 13 colonies that grew in liquid LB medium with Amp. (The method we used was without columns because the kit ran out)....)
- 23:19, 21 October 2010 (diff | hist) Team:Panama/2 August 2010 (→August 02)
- 23:18, 21 October 2010 (diff | hist) Team:Panama/30 July 2010 (→July 30)
- 23:13, 21 October 2010 (diff | hist) Team:Panama/27 July 2010 (→July 27)
- 23:08, 21 October 2010 (diff | hist) N Team:Panama/13 October 2010 (New page: Quick Screening Protocol: 100 ul of overnight culture 50 ul Phenol:Chloroform:Isoamyl alcohol (25:24:1) 10 ul Loading Buffer We loaded 20 ul into each well Preparation of the Primers f...)
- 23:01, 21 October 2010 (diff | hist) Team:Panama/2 August 2010
- 23:00, 21 October 2010 (diff | hist) N Team:Panama/2 August 2010 (New page: {{:Team:Panama/calendar2}})
- 22:54, 21 October 2010 (diff | hist) N Team:Panama/12 October 2010 (New page: We did a transformation with the pET28a to test if the JM109 competent cells that just arrived are still viable after being transported in an inadequate manner. We inoculated 6 colonies i...)
- 22:51, 21 October 2010 (diff | hist) N Team:Panama/11 October 2010 (New page: Electrophoresis of the ligation product: Terminator, GFP 1% agarose gel, TBE 1X, 2.5 ul EtBr' *Photo 3055 PsB1AC3 151 Terminator 742 GFP =3948 bp)
- 22:49, 21 October 2010 (diff | hist) N Team:Panama/8 October 2010 (New page: Concentration Measurement of the purified PCR product 107ng/ul Proportional Calculation for repeating the cloning with pGEM-T Easy Vector System: Insert:Vector molar ratios Recommended ...)
- 22:43, 21 October 2010 (diff | hist) N Team:Panama/7 October 2010 (New page: We did the miniprep of the PsB1C3 plasmid We loaded it into a 1% agarose gel)
- 22:41, 21 October 2010 (diff | hist) N Team:Panama/6 October 2010 (New page: We did colony PCR of the cloning plate. We tested 20 white colonies to test if they have the insert (Rh1ab). We also did the transformation of the plasmid with chloranfenicol PsB1C3 in or...)
- 22:35, 21 October 2010 (diff | hist) Team:Panama/4 October 2010 (→October 4)
- 22:30, 21 October 2010 (diff | hist) Team:Panama/29 July 2010 (→July 29)
- 22:28, 21 October 2010 (diff | hist) N Team:Panama/4 October 2010 (New page: Ligation of Reporter and Terminator (About two hours) We used the same quantities that were used on September 23 2010 PCR (About 2.5 hours) On October 1, we did the miniprep of the clo...)
- 21:42, 21 October 2010 (diff | hist) Team:Panama/27 July 2010 (→July 27)
- 21:40, 21 October 2010 (diff | hist) Team:Panama/28 July 2010 (→July 28)
- 21:39, 21 October 2010 (diff | hist) Team:Panama/28 July 2010 (→July 28)
- 21:14, 21 October 2010 (diff | hist) Team:Panama/27 July 2010 (→July 27)
- 20:58, 21 October 2010 (diff | hist) Team:Panama/28 September 2010
- 20:56, 21 October 2010 (diff | hist) N Team:Panama/28 September 2010 (New page: Digestion of Rh1ab with Pst1 -Electrophoresis Clone Rh1ab into the plasmid -This week PCR to show the RH1ab gene from within the plasmid it was cloned into Mutagenesis (Next week) Th...)
- 20:46, 21 October 2010 (diff | hist) N Team:Panama/24 September 2010 (24 Sept 2010)
- 20:45, 21 October 2010 (diff | hist) Team:Panama/23 September 2010
- 20:45, 21 October 2010 (diff | hist) N Team:Panama/23 September 2010 (23 Sept 2010)
- 20:44, 21 October 2010 (diff | hist) N Team:Panama/22 September 2010 (22 Sept 2010)
- 20:42, 21 October 2010 (diff | hist) N Team:Panama/16 September 2010 (16 Sept 2010)
- 20:36, 21 October 2010 (diff | hist) N Team:Panama/15 September 2010 (New page: Well 23L. BBa_B0015 Plasmid pSB1AK3 -Insert 151 bp -Plasmid 3168 bp)
- 20:34, 21 October 2010 (diff | hist) Team:Panama/26 July 2010 (→July 26)
- 20:27, 21 October 2010 (diff | hist) Team:Panama/21 July 2010 (→Meeting July 21)
- 20:26, 21 October 2010 (diff | hist) Team:Panama/21 July 2010 (→Meeting July 21)
- 20:23, 21 October 2010 (diff | hist) Team:Panama/20 July 2010 (→July 20)
- 21:54, 23 September 2010 (diff | hist) Team:Panama/Team/students (→Lauris)
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