Team:BCCS-Bristol/Wetlab/0-10mM Assay
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(New page: ==Characterising the Biobrick - 0 - 10mM assay== Having established the biobrick was definitely switched on by 20mM of KNO3, we wanted to conduct a broader test of its behaviour across a ...)
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(New page: ==Characterising the Biobrick - 0 - 10mM assay== Having established the biobrick was definitely switched on by 20mM of KNO3, we wanted to conduct a broader test of its behaviour across a ...)
Newer edit →
Revision as of 09:32, 13 October 2010
Characterising the Biobrick - 0 - 10mM assay
Having established the biobrick was definitely switched on by 20mM of KNO3, we wanted to conduct a broader test of its behaviour across a range of different nitrate levels
An assay was set up between 0 – 10 mM of Potassium Nitrate at 2mM intervals. The same colony of MG1655 containing BBa_K381001 was used to inoculate six 5mL tubes of LB at varying nitrate levels as listed below. This was repeated twice to allow the results to be averaged