Team:UNIPV-Pavia/Calendar/October
From 2010.igem.org
Line 72: | Line 72: | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 1</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 4th|October, 4th]]</td><td align="left"> | ||
- | 104° Bio-Lab -Miniprep and quantification of: INTEIN_C3, I74-8, I54, I35. Digest and gel run/cut/quantification of these samples. | + | 104° Bio-Lab - Miniprep and quantification of: INTEIN_C3, I74-8, I54, I35. Digest and gel run/cut/quantification of these samples. |
Ligations of I78=I74+INTEIN_C3. | Ligations of I78=I74+INTEIN_C3. | ||
Line 79: | Line 79: | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 5th|October, 5th]]</td><td align="left"> | ||
- | 105° Bio-Lab -Digestions were performed again for I55, I37, I57, I31 and I54. Digestions were let at +37°C ON. | + | 105° Bio-Lab - Digestions were performed again for I55, I37, I57, I31 and I54. Digestions were let at +37°C ON. |
Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | Transformation (1ul) of I78 into E. coli TOP10. In order to check our E. coli TOP10 competent cells we plated them on LB+Amp and Cm agar plates. | ||
Line 86: | Line 86: | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 6th|October, 6th]]</td><td align="left"> | ||
- | 106° Bio-Lab -E. coli TOP10 plated on LB+Amp agar plate showed red colonies!!! | + | 106° Bio-Lab - E. coli TOP10 plated on LB+Amp agar plate showed red colonies!!! |
Transformation of available ligations I75 and I77 (1ul) into E. coli TOP10. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | Transformation of available ligations I75 and I77 (1ul) into E. coli TOP10. They were plated on LB+Amp agar plates and let grow ON at +37°C, 220 rpm. | ||
Line 95: | Line 95: | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 7th|October, 7th]]</td><td align="left"> | ||
- | 107° Bio-Lab -I78 screening gel run. Glycerol stock and miniprep for I78-3. | + | 107° Bio-Lab - I78 screening gel run. Glycerol stock and miniprep for I78-3. |
Colony PCR for screening of I79 (we pick eleven colonies). | Colony PCR for screening of I79 (we pick eleven colonies). | ||
Line 104: | Line 104: | ||
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 8th|October, 8th]]</td><td align="left"> | ||
- | 108° Bio-Lab - | + | 108° Bio-Lab - Glycerol stock, miniprep and Nanodrop quantification of I79-2.This DNA was sent to BMR Genomics for sequencing. |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 9th|October, 9th]]</td><td align="left"> | ||
109° Bio-Lab - | 109° Bio-Lab - | ||
+ | </td></tr> | ||
+ | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana1 #October, 10th|October, 10th]]</td><td align="left"> | ||
+ | 110° Bio-Lab - Inoculum into 4 ml LB+Amp of: I75-1/2/3, I76-1/2, I77-1/2/3, I47, I48, I49, I63, I64 and I65.They were let grow at +37°C, 220 rpm. | ||
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 2</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 11th|October, 11th]]</td><td align="left"> | ||
- | + | 111° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 12th|October, 12th]]</td><td align="left"> | ||
- | + | 112° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 13th|October, 13th]]</td><td align="left"> | ||
- | + | 113° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 14th|October, 14th]]</td><td align="left"> | ||
- | + | 114° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 15th|October, 15th]]</td><td align="left"> | ||
- | + | 115° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana2 #October, 16th|October, 16th]]</td><td align="left"> | ||
- | + | 116° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 3</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 18th|October, 18th]]</td><td align="left"> | ||
- | + | 117° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 19th|October, 19th]]</td><td align="left"> | ||
- | + | 118° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 20th|October, 20th]]</td><td align="left"> | ||
- | + | 119° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 21st|October, 21st]]</td><td align="left"> | ||
- | + | 120° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 22nd|October, 22nd]]</td><td align="left"> | ||
- | + | 121° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana3 #October, 23rd|October, 23rd]]</td><td align="left"> | ||
- | + | 122° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>OCTOBER: WEEK 4</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 25th|October, 25th]]</td><td align="left"> | ||
- | + | 123° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 26th|October, 26th]]</td><td align="left"> | ||
- | + | 124° Bio-Lab - | |
</td></tr> | </td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left"> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/October/settimana4 #October, 27th|October, 27th]]</td><td align="left"> | ||
- | + | 125° Bio-Lab - Closing wiki | |
</td></tr> | </td></tr> | ||
Revision as of 19:24, 10 October 2010
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|