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Team:Harvard/allergy/parts
From 2010.igem.org
(Difference between revisions)
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td></b></td><td><b>Sequence</b></td></tr> | <tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td></b></td><td><b>Sequence</b></td></tr> | ||
<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td></tr> | <tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td></tr> | ||
| - | <tr><td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td></td></tr> | + | <tr> "<a href="insert file name">amiRNA GFP</a>"<td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td> "<a href="insert">amiRNA x</a>"</td></tr> |
| - | <tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td></td></tr> | + | <tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td>"<a href="insert file name">ihpRNA Bet</a>"</td></tr> |
| - | <tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td></td></tr> | + | <tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td>"<a href="insert file name">ihpRNA LTP</a>"</td></tr> |
| - | <tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td></td></tr> | + | <tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td>"<a href="insert file name">ihpRNA GER</a>"</td></tr> |
</table> | </table> | ||
<br /><br /> | <br /><br /> | ||
Revision as of 16:23, 10 October 2010
parts and primers
parts and primers
Allergy
For more information on the vectors visit our "meet the allergens" page| Name | Original Name | Description | Resistance | Sequence | |
| A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | ||
| A2 | amiRNA (Other allergen x) | knockdown construct for x | chloramphenicol | "amiRNA x" | |
| A3 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | "ihpRNA Bet" | |
| A4 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | "ihpRNA LTP" | |
| A5 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | "ihpRNA GER" |
Primers
| Name | Description | Sequence |
| VP3 | HindIII-pENTCUP2 forward primer for Expression Series insert | |
| VP4 | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | |
| VP5 | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
| VP6 | gusA-NheI reverse primer for Reporter Series 1 insert | |
| VP7 | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
| VP8 | smGFP-NheI reverse primer for Reporter Series 1 insert | |
| VP9 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP11 | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
| VP12 | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP13 | 2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP14 | 3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP15 | 4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP16 | 5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter | |
| VP17 | 1 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP18 | 2 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP19 | 3 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP20 | 4 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP21 | 5 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP22 | 6 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP23 | 7 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP24 | 8 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP25 | 9 of 9 for sequencing V11 biobrick cloning site and gusA reporter | |
| VP26 | 1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP27 | 2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP28 | 3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP29 | 4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP30 | 5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter | |
| VP33 | 1 of 2 for sequencing V7 and V8 biobrick cloning site | |
| VP34 | 2 of 2 for sequencing V7 and V8 biobrick cloning site |
