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Team:Michigan/Protocols
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[[Media:8-10-2010_Ligation_Calculations_updated.pdf|Updated T4 DNA Ligase Protocol (Not quick ligase)]] | [[Media:8-10-2010_Ligation_Calculations_updated.pdf|Updated T4 DNA Ligase Protocol (Not quick ligase)]] | ||
| + | *For additional information/questions, refer to [http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/ligation_tips.asp NEB Ligation Tips] | ||
*[[Media:Ligation_Protocol.pdf|Ligation]] | *[[Media:Ligation_Protocol.pdf|Ligation]] | ||
**Ginko Bioworks Protocol | **Ginko Bioworks Protocol | ||
Revision as of 14:38, 8 October 2010
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Epifluorescence Microscope Usage - H.H. Dow
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Adopted from Jeremy Minty's protocol
- General protocol with specific instructions for ERB/Lin lab equipment
Updated T4 DNA Ligase Protocol (Not quick ligase)
- For additional information/questions, refer to [http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/ligation_tips.asp NEB Ligation Tips]
- Ligation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
Group-Specific Protocols
Oil Sands
Revised biofilm assay protocol
Pili
Quorum Sensing
General Outline for Biobrick Manipulations
