Team:Groningen/1 June 2010

From 2010.igem.org

(Difference between revisions)
Line 17: Line 17:
Template 0.5 ~14ng
Template 0.5 ~14ng
Taq 0.5 2.5u
Taq 0.5 2.5u
-
MQ 29 </pre
+
MQ 29 </pre>
- 94°C, 3’
- 94°C, 3’

Revision as of 21:36, 7 October 2010

iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

We've started working on the wiki, been preparing plasmids and are settling in our office space. Note to self: should have brought coffee machine


Week 22

The biobrick prefix and suffix are introduced into the pNZ8901 expression vector using PCR and primers with 5’ overhangs.

For optimization taq polymerase (fermentas) is used first

Component	µl	Final concentration
Primer pNZ89bbs-for1	5	300nM
Primer pNZ89bbs-rev1	5	300nM
10x Taq buffer(-MgCl2)	5	1x
dNTP’s	2	200µM
MgCl2	3	1.5mM
Template	0.5	~14ng
Taq	0.5	2.5u
MQ	29	

- 94°C, 3’ - 94°C, 30’’ 30X - 50°C, 30’’ - 72°C, 1’30’’ - 72°C, 10’

alt text











Week 23











Search

 

iGEM 2010 main page
iGEM HQ
iGEM Groningen Team page

University of Groningen
Where on earth are we?
Share |