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Team:Groningen/1 June 2010
From 2010.igem.org
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The biobrick prefix and suffix are introduced into the pNZ8901 expression vector using PCR and primers with 5’ overhangs. | The biobrick prefix and suffix are introduced into the pNZ8901 expression vector using PCR and primers with 5’ overhangs. | ||
Revision as of 21:27, 7 October 2010
Hydrophobofilm
pushing coatings into a greener future
We've started working on the wiki, been preparing plasmids and are settling in our office space. Note to self: should have brought coffee machine
Week 22
The biobrick prefix and suffix are introduced into the pNZ8901 expression vector using PCR and primers with 5’ overhangs.
For optimization taq polymerase (fermentas) is used first Component µl Final concentration Primer pNZ89bbs-for1 5 300nM Primer pNZ89bbs-rev1 5 300nM 10x Taq buffer(-MgCl2) 5 1x dNTP’s 2 200µM MgCl2 3 1.5mM Template 0.5 ~14ng Taq 0.5 2.5u MQ 29
- 94°C, 3’ - 94°C, 30’’ 30X - 50°C, 30’’ - 72°C, 1’30’’ - 72°C, 10’
Week 23
