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Team:Groningen/Transformation to E. coli
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(New page: '''Transformation to ''E. coli''''' *Add 10 µL of ligate to 100 µL competent cells *Put cells for 30 minutes on ice *Heat shock the cells for 45 seconds at 42 °C *Put cells on ice for ...)
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(New page: '''Transformation to ''E. coli''''' *Add 10 µL of ligate to 100 µL competent cells *Put cells for 30 minutes on ice *Heat shock the cells for 45 seconds at 42 °C *Put cells on ice for ...)
Newer edit →
Revision as of 20:46, 7 October 2010
Transformation to E. coli
- Add 10 µL of ligate to 100 µL competent cells
- Put cells for 30 minutes on ice
- Heat shock the cells for 45 seconds at 42 °C
- Put cells on ice for 2 minutes
- Add 400 µL TY and grow for 1 hour at 37 °C
- Plate 100 µL of cells on LB agar (2%) plates
