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Team:DTU-Denmark
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<img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="570px" align="center"> </img> | <img src="https://static.igem.org/mediawiki/2010/f/ff/DTU_Project_illustration_1.png" width="570px" align="center"> </img> | ||
<p align="left"><font size="1">Figure 1</font></p><br> | <p align="left"><font size="1">Figure 1</font></p><br> | ||
| - | <p align="justify">Figure 1 shows the basic concept of our project: <i>Inducer 1</i> will activate <i>Promoter 1</i> thereby start up the production of <i>Repressor 1</i>, switching off the other part of our switch by repressing <i>Promoter 2</i>. This will essentially make the cells change from <font color="#990000">red</font color> to <font color="#33FF00">green</font color>!</p> | + | <p align="justify">Figure 1 shows the basic concept of our project:<br><i>Inducer 1</i> will activate <i>Promoter 1</i> thereby start up the production of <i>Repressor 1</i>, switching off the other part of our switch by repressing <i>Promoter 2</i>. This will essentially make the cells change from <font color="#990000">red</font color> to <font color="#33FF00">green</font color>!</p> |
<p align="justify">This means that when the cells are exposed to <i>Inducer 2</i>, the cell culture will turn from <font color="#33FF00">green</font color> to <font color="#990000">red</font color>.</p> | <p align="justify">This means that when the cells are exposed to <i>Inducer 2</i>, the cell culture will turn from <font color="#33FF00">green</font color> to <font color="#990000">red</font color>.</p> | ||
</font> | </font> | ||
Revision as of 20:00, 4 October 2010
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Sponsors and acknowledgements - List of sponsors - Acknowledgements Also check out our Danish website - DTU iGEM 2010 Also check us out on: |
DTU DENMARK The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems. We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system have been modeled and we have also attempted to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks. Basic Concept
Figure 1 Figure 1 shows the basic concept of our project: This means that when the cells are exposed to Inducer 2, the cell culture will turn from green to red. |
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