Team:DTU-Denmark

From 2010.igem.org

(Difference between revisions)

Revision as of 10:48, 4 October 2010

Welcome to the DTU iGEM wiki!

Notebook

Sponsors and acknowledgements

- List of sponsors
- Acknowledgement

Also check out our Danish website

- DTU iGEM 2010

Also check us out on:

- Facebook
- Twitter

DTU DENMARK

The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.

We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system have been modeled and we have also attempted to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.

We have hereby demonstrated the engineering of a multipurpose bi-stable switch sensor/reporter tool that can have numerous applications.

Quick Update

August 14th, 2010
Pictures have been posted so check out our picture gallery!

Comments or questions to the team? Please Email us -- Comments of questions to webmaster? Please Email us

@@ Line 170: / Line 170: @@
     <font color="DDDDDD" face="arial" size="2">
      <a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>- DTU iGEM 2010</a><br>
+    <br>
+   </font>
+<font color="#990000" face="arial" size="3">
+<br>
+ Also check us out on:<br><br>
+   </font>
+   <font color="DDDDDD" face="arial" size="2">
+    <a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>- Facebook</a><br>
+   </font>
+   <font color="DDDDDD" face="arial" size="2">
+    <a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>- Twitter</a><br>
      <br>
     </font>
@@ Line 183: / Line 194: @@
 <!-- INSERT MAIN TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
-<p align="justify">Greetings from Team DTU!<br>
+<p align="justify">The aim of this project is to engineer a genetic bi-stable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.
-Our team is made up of 10 students competing in this years iGEM competition. This year's team representing <a href="http://www.dtu.dk/English/About_DTU.aspx">DTU</a> is made up of a diverse group of students, both culturally and scientifically. This means that we get to learn so much from each other, professionally as well as culturally. As we are studying at a technical University, we are given an even greater opportunity to really flex our brain-muscles and be innovative. <i>You can also follow us on <b><a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts">Facebook</a></b> and <b><a href="http://twitter.com/igemdtu2010">Twitter</a></b>!</i>
+<p align="justify">We present the framework for this development and characterize the regulatory mechanisms by using fluorescent proteins as the reporter (outputs). The dynamics of the system have been modeled and we have also attempted to characterize and submit the promoters, repressors and anti-repressors from the salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.
+<p align="justify">We have hereby demonstrated the engineering of a multipurpose bi-stable switch sensor/reporter tool that can have numerous applications.
-<p align="justify">The goal of our project is to enable colonies of E. coli bacteria to transition between production of two different reporter proteins. In our system, switching between states will be induced by exposing the bacteria to light. Each of the states will have a specific frequency associated with it. There are multiple potential applications for biologicals "switches" such as these, this includes the improved control of production of additives in industrial biotechnological processes. For more details, please click <a href="https://2010.igem.org/wiki/Team:DTU-Denmark/Project">here</a>.
-<p align="justify"><img src="https://static.igem.org/mediawiki/2010/7/78/DTU_paris.jpg" width="570px"  align="left"> The team in Paris for the iGEM workshop (Malte is missing)</img>
    </font>
   </td>