Team:HokkaidoU Japan/Notebook/October3

From 2010.igem.org

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<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/October2|October 2]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/October4|October 4]]</div></div>
<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/October2|October 2]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/October4|October 4]]</div></div>
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==Miniprep==
 +
[[Image:HokkaidoU Japan 20101003a.JPG|200px|right|thumb]]
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コロニー8, 10, 11, 12, 13, 14, 15, 16からミニプレキットでプラスミドを精製した<br>
 +
->うち1 uLを泳動して確認
 +
* Insertはおよそ1800 bp, Vectorはおよそ2500 b
 +
* コントロールの16番以外，それらしいバンドがある
 +
* 2 ug/uLくらいのDNA量と推定
 +
==Insertの確認==
 +
[[Image:HokkaidoU Japan 20101003b.JPG|200px|right|thumb]]
 +
Miniprepで得たプラスミドをNot Iで処理すれば，約1800 bpのInsertが得られるはずである
 +
{|class="protocol" style="text-align:center;"
 +
!well
 +
!DNA
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|-
 +
|1
 +
|λ/Hind III (2 uL)
 +
|-
 +
|2
 +
|pSB1T3 (1 uL)
 +
|-
 +
|3
 +
|No.8/Not I
 +
|-
 +
|4
 +
|No.8/EcoR I
 +
|-
 +
|5
 +
|No.8/intact
 +
|-
 +
|6
 +
|No.10/Not I
 +
|-
 +
|7
 +
|No.10/EcoR I
 +
|-
 +
|8
 +
|No.10/intact
 +
|-
 +
|9
 +
|No.11/Not I
 +
|-
 +
|10
 +
|No.11/EcoR I
 +
|-
 +
|11
 +
|No.11/intact
 +
|-
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|12
 +
|No.14/Not I
 +
|-
 +
|13
 +
|No.14/EcoR I
 +
|-
 +
|14
 +
|No.14/intact
 +
|-
 +
|15
 +
|No.16/Not I
 +
|-
 +
|16
 +
|No.16/EcoR I
 +
|-
 +
|17
 +
|No.16/intact
 +
|}

Revision as of 06:31, 4 October 2010

Notebook

Miniprep

HokkaidoU Japan 20101003a.JPG

コロニー8, 10, 11, 12, 13, 14, 15, 16からミニプレキットでプラスミドを精製した
->うち1 uLを泳動して確認

  • Insertはおよそ1800 bp, Vectorはおよそ2500 b
  • コントロールの16番以外，それらしいバンドがある
  • 2 ug/uLくらいのDNA量と推定

Insertの確認

HokkaidoU Japan 20101003b.JPG

Miniprepで得たプラスミドをNot Iで処理すれば，約1800 bpのInsertが得られるはずである

well DNA
1 λ/Hind III (2 uL)
2 pSB1T3 (1 uL)
3 No.8/Not I
4 No.8/EcoR I
5 No.8/intact
6 No.10/Not I
7 No.10/EcoR I
8 No.10/intact
9 No.11/Not I
10 No.11/EcoR I
11 No.11/intact
12 No.14/Not I
13 No.14/EcoR I
14 No.14/intact
15 No.16/Not I
16 No.16/EcoR I
17 No.16/intact
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/October3"