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Team:HokkaidoU Japan/Notebook/August31
From 2010.igem.org
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| - | =Gel extraction of pUC119, Ligation and Transformation= | + | =[[Team:HokkaidoU_Japan/Protocols|Gel extraction]] of pUC119, Ligation and [[Team:HokkaidoU_Japan/Protocols|Transformation]]= |
* Added 10 uL 6x Sample Buffer to pUC119 solution 50 uL | * Added 10 uL 6x Sample Buffer to pUC119 solution 50 uL | ||
* Divided among 3 lanes | * Divided among 3 lanes | ||
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|style="border-top:1px solid #996;"|4.4 uL | |style="border-top:1px solid #996;"|4.4 uL | ||
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| - | + | →[[Team:HokkaidoU_Japan/Protocols|Transformation]] | |
Latest revision as of 09:13, 2 October 2010
since 13 Jul 2010
since 1 Aug 2010
Gel extraction of pUC119, Ligation and Transformation
- Added 10 uL 6x Sample Buffer to pUC119 solution 50 uL
- Divided among 3 lanes
- Gel extraction (gel weight: 220 mg)
- Final volume after extraction was50 uL（500 ng/uL）
Ligation
| Empty | I | M | |
| DNA | 2 uL | 2 uL | 2 uL |
| ligation solution I | － | 2 uL | － |
| Mighty Mix | － | － | 2 uL |
| T4 ligase | － | 0.4 uL | 0.4 uL |
| Total | 2 uL | 4.4 uL | 4.4 uL |
