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Team:Freiburg Bioware/NoteBook/Labjournal/October
From 2010.igem.org
(→mini preps of CD clones) |
(→136. labday 01.10.2010) |
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| align="left" | BSA (10x) ||align="left"| 2 µl | | align="left" | BSA (10x) ||align="left"| 2 µl | ||
|- | |- | ||
| - | | align="left" | Buffer no. 4 | + | | align="left" | Buffer no. 4 ||align="left"| 2,0 µl |
|- | |- | ||
| align="left" | Enzyme 1 XbaI ||align="left"| 1,0 µl | | align="left" | Enzyme 1 XbaI ||align="left"| 1,0 µl | ||
| Line 36: | Line 36: | ||
[[Image: Freiburg10_CD testdigestion 2010-10-01.jpg]] | [[Image: Freiburg10_CD testdigestion 2010-10-01.jpg]] | ||
| + | |||
| + | ====<p style="font-size:15px; background-color:#66bbff;"><b>Biobrick assembly pSB1C3_lITR_hTERT_beta-globin_CD</b></p>==== | ||
| + | '''Investigator: Kira''' | ||
| + | |||
| + | c(pSB1C3_lITR_hTERT_beta-globin)= 333 ng/ul | ||
| + | c(pSB1C3_CD)= 151 ng/ul | ||
| + | |||
| + | | border="1" | ||
| + | | align="left" | '''Components''' ||align="left"| <b>vector</b> Volume/µL ||align="left"| <b>insert</b> Volume/µL | ||
| + | |- | ||
| + | | align="left" | DNA ||align="left"| 4,5 µl||align="left"| 6 | ||
| + | |- | ||
| + | | align="left" | BSA (10x) ||align="left"| 3 µl||align="left"| 3 | ||
| + | |- | ||
| + | | align="left" | Buffer no. 4 ||align="left"| 3,0 µl||align="left"| 3 | ||
| + | |- | ||
| + | | align="left" | Enzyme 1 XbaI ||align="left"| 0 µl||align="left"|1,5 | ||
| + | |- | ||
| + | | align="left" | Enzyme 2 SpeI ||align="left"| 1,5 µl||align="left"| 0 | ||
| + | |- | ||
| + | | align="left" | Enzyme 3 PstI-HF ||align="left"| 1,0 ||align="left"| 1 | ||
| + | |- | ||
| + | | align="left" | H<sub>2</sub>O ||align="left"| 17||align="left"| 15,5 | ||
| + | |- | ||
| + | | align="left" | '''Total volume''' ||align="left"| <b>25</b> | ||
| + | |} | ||
| + | <br /> | ||
| + | |||
| + | incubation @ 37 C for approx. 2 h | ||
| + | |||
| + | 1% agarose gel <br /> | ||
Revision as of 17:23, 1 October 2010
Contents |
136. labday 01.10.2010
mini preps of CD clones
Investigator: Kira
c(p689)= 115 ng/ul
c(690)= 151, 20 ng/ul
c(691)= 122,27 ng/ul
c(p692) = 126,42 ng/ul
test digestion of CD clones
Investigator: Kira
| Components | sample Volume/µL |
| DNA | 4,0 µl |
| BSA (10x) | 2 µl |
| Buffer no. 4 | 2,0 µl |
| Enzyme 1 XbaI | 1,0 µl |
| Enzyme 2 AgeI | 1,5 µl |
| H2O | 9,5 µl |
| Total volume | 20 |
incubation @ 37 C for approx. 2 h
1% agarose gel
Biobrick assembly pSB1C3_lITR_hTERT_beta-globin_CD
Investigator: Kira
c(pSB1C3_lITR_hTERT_beta-globin)= 333 ng/ul c(pSB1C3_CD)= 151 ng/ul
| border="1"
| align="left" | Components ||align="left"| vector Volume/µL ||align="left"| insert Volume/µL
|-
| align="left" | DNA ||align="left"| 4,5 µl||align="left"| 6
|-
| align="left" | BSA (10x) ||align="left"| 3 µl||align="left"| 3
|-
| align="left" | Buffer no. 4 ||align="left"| 3,0 µl||align="left"| 3
|-
| align="left" | Enzyme 1 XbaI ||align="left"| 0 µl||align="left"|1,5
|-
| align="left" | Enzyme 2 SpeI ||align="left"| 1,5 µl||align="left"| 0
|-
| align="left" | Enzyme 3 PstI-HF ||align="left"| 1,0 ||align="left"| 1
|-
| align="left" | H2O ||align="left"| 17||align="left"| 15,5
|-
| align="left" | Total volume ||align="left"| 25
|}
incubation @ 37 C for approx. 2 h
1% agarose gel
