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Revision as of 09:12, 1 October 2010 (view source) Kira M (Talk | contribs) (→mini preps of CD clones) ← Older edit		Revision as of 17:16, 1 October 2010 (view source) Kira M (Talk | contribs) (→test digestion of CD clones) Newer edit →
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	1% agarose gel <br />		1% agarose gel <br />
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Revision as of 17:16, 1 October 2010

136. labday 01.10.2010

mini preps of CD clones

Investigator: Kira

c(p689)= 115 ng/ul
c(690)= 151, 20 ng/ul
c(691)= 122,27 ng/ul
c(p292) = 126,42 ng/ul

test digestion of CD clones

Investigator: Kira

Components	sample Volume/µL
DNA	4,0 µl
BSA (10x)	2 µl
Buffer no. 4 (10x)	2,0 µl
Enzyme 1 XbaI	1,0 µl
Enzyme 2 AgeI	1,5 µl
H2O	9,5 µl
Total volume	20

incubation @ 37 C for approx. 2 h

1% agarose gel