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Team:Freiburg Bioware/NoteBook/Labjournal/October
From 2010.igem.org
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'''Investigator: Kira''' | '''Investigator: Kira''' | ||
| - | c(p689)= 115 ng/ul | + | c(p689)= 115 ng/ul <br /> |
| - | c(690)= 151, 20 ng/ul | + | c(690)= 151, 20 ng/ul <br /> |
| - | c(691)= 122,27 ng/ul | + | c(691)= 122,27 ng/ul <br /> |
| - | c(p292) = 126,42 ng/ul | + | c(p292) = 126,42 ng/ul <br /> |
<br /> | <br /> | ||
Revision as of 09:12, 1 October 2010
136. labday 01.10.2010
mini preps of CD clones
Investigator: Kira
c(p689)= 115 ng/ul
c(690)= 151, 20 ng/ul
c(691)= 122,27 ng/ul
c(p292) = 126,42 ng/ul
test digestion of CD clones
Investigator: Kira
| Components | sample Volume/µL |
| DNA | 4,0 µl |
| BSA (10x) | 2 µl |
| Buffer no. 4 (10x) | 2,0 µl |
| Enzyme 1 XbaI | 1,0 µl |
| Enzyme 2 AgeI | 1,5 µl |
| H2O | 9,5 µl |
| Total volume | 20 |
incubation @ 37 C for approx. 2 h
1% agarose gel
