Team:Freiburg Bioware/NoteBook/Labjournal/October
From 2010.igem.org
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| align="left" | Enzyme 2 AgeI ||align="left"| 1,5 µl | | align="left" | Enzyme 2 AgeI ||align="left"| 1,5 µl | ||
|- | |- | ||
- | | align="left" | H<sub>2</sub>O ||align="left"| | + | | align="left" | H<sub>2</sub>O ||align="left"| 9,5 µl |
|- | |- | ||
| align="left" | '''Total volume''' ||align="left"| <b>20</b> | | align="left" | '''Total volume''' ||align="left"| <b>20</b> |
Revision as of 09:12, 1 October 2010
136. labday 01.10.2010
mini preps of CD clones
Investigator: Kira
c(p689)= 115 ng/ul
c(690)= 151, 20 ng/ul
c(691)= 122,27 ng/ul
c(p292) = 126,42 ng/ul
test digestion of CD clones
Investigator: Kira
Components | sample Volume/µL |
DNA | 4,0 µl |
BSA (10x) | 2 µl |
Buffer no. 4 (10x) | 2,0 µl |
Enzyme 1 XbaI | 1,0 µl |
Enzyme 2 AgeI | 1,5 µl |
H2O | 9,5 µl |
Total volume | 20 |
incubation @ 37 C for approx. 2 h
1% agarose gel