Team:Freiburg Bioware/NoteBook/Labjournal/October

From 2010.igem.org

(Difference between revisions)
(136. labday 01.10.2010)
(test digestion of CD clones)
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| align="left" | Enzyme 2 AgeI  ||align="left"| 1,5 µl
| align="left" | Enzyme 2 AgeI  ||align="left"| 1,5 µl
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|-
-
| align="left" | H<sub>2</sub>O ||align="left"| 7,5 µl
+
| align="left" | H<sub>2</sub>O ||align="left"| 9,5 µl
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| align="left" | '''Total volume''' ||align="left"| <b>20</b>   
| align="left" | '''Total volume''' ||align="left"| <b>20</b>   

Revision as of 09:12, 1 October 2010

136. labday 01.10.2010

mini preps of CD clones

Investigator: Kira

c(p689)= 115 ng/ul c(690)= 151, 20 ng/ul c(691)= 122,27 ng/ul c(p292) = 126,42 ng/ul

test digestion of CD clones

Investigator: Kira

Components sample Volume/µL
DNA 4,0 µl
BSA (10x) 2 µl
Buffer no. 4 (10x) 2,0 µl
Enzyme 1 XbaI 1,0 µl
Enzyme 2 AgeI 1,5 µl
H2O 9,5 µl
Total volume 20


incubation @ 37 C for approx. 2 h

1% agarose gel