Team:Freiburg Bioware/NoteBook/Labjournal/October

From 2010.igem.org

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===<p style="font-size:17px; background-color:#00dd77;">136. labday 01.10.2010</p>===
===<p style="font-size:17px; background-color:#00dd77;">136. labday 01.10.2010</p>===
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====<p style="font-size:15px; background-color:#66bbff;"><b>mini preps of CD clones</b></p>====
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'''Investigator: Kira'''
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 +
c(p689)= 115 ng/ul
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c(690)= 151, 20 ng/ul
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c(691)= 122,27 ng/ul
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c(p292) = 126,42 ng/ul
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<br />
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====<p style="font-size:15px; background-color:#66bbff;"><b>test digestion of CD clones</b></p>====
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'''Investigator: Kira'''
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{| border="1"
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| align="left" | '''Components''' ||align="left"| <b>sample</b> Volume/µL
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|-
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| align="left" | DNA  ||align="left"| 4,0 µl
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|-
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| align="left" | BSA (10x) ||align="left"| 2 µl
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|-
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| align="left" | Buffer no. 4 (10x) ||align="left"| 2,0 µl
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|-
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| align="left" | Enzyme 1 XbaI ||align="left"| 1,0 µl
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|-
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| align="left" | Enzyme 2 AgeI  ||align="left"| 1,5 µl
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|-
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| align="left" | H<sub>2</sub>O ||align="left"| 7,5 µl
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|-
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| align="left" | '''Total volume''' ||align="left"| <b>20</b> 
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|}
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<br />
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incubation @ 37 C for approx. 2 h
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1% agarose gel <br />

Revision as of 09:11, 1 October 2010

136. labday 01.10.2010

mini preps of CD clones

Investigator: Kira

c(p689)= 115 ng/ul c(690)= 151, 20 ng/ul c(691)= 122,27 ng/ul c(p292) = 126,42 ng/ul

test digestion of CD clones

Investigator: Kira

Components sample Volume/µL
DNA 4,0 µl
BSA (10x) 2 µl
Buffer no. 4 (10x) 2,0 µl
Enzyme 1 XbaI 1,0 µl
Enzyme 2 AgeI 1,5 µl
H2O 7,5 µl
Total volume 20


incubation @ 37 C for approx. 2 h

1% agarose gel

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