Team:UCL London/Digestion

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Revision as of 14:06, 30 September 2010

UCL IGEM 2010

RETURN TO IGEM 2010

Restriction Enzyme Digestion

Materials

Plasmid DNA: 10.5 µL/5 µL

Restriction Enzymes (depend on the requirements of the parts)

  EcoRI: 1 µL
  PstI: 1 µL
  SpeI: 1 µL
  XbaI: 1 µL
  10× NE buffer: 1.5 µL/1 µL (lid)
  BSA: 1 µL (lid)

Method

1. Add all the contents listed above to labelled eppendorfs.

2. Spin for 30 sec.

3. Incubation at 37°C for 1hr.

4. Leave on ice before the gel electrophoresis


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