Team:Cambridge/Gibson/Protocol

From 2010.igem.org

(Difference between revisions)
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== Master mix ==
== Master mix ==
-
Ingredients
+
 
 +
{|class="wikitable"
 +
|-
 +
|
 +
|Volume/µl
 +
|-
 +
|Taq ligase 40u/µl
 +
|50
 +
|-
 +
|5x isothermal buffer
 +
|100
 +
|-
 +
|T5 exonuclease 1u/µl
 +
|2
 +
|-
 +
|Phusion polymerase 2u/µl
 +
|6.25
 +
|-
 +
|Nuclease--free water
 +
|216.75
 +
|-
 +
|
 +
|=375
 +
|}
{{:Team:Cambridge/Templates/footer}}
{{:Team:Cambridge/Templates/footer}}

Revision as of 09:54, 30 September 2010

Retrieved from "http://2010.igem.org/Team:Cambridge/Gibson/Protocol"