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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/11
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
| - | === | + | ===7:Electrophoresis=== |
<Member><br /> | <Member><br /> | ||
Mariko,mizuki | Mariko,mizuki | ||
Revision as of 07:09, 28 September 2010
Contents |
1:PCR
<Member>
mio,easily
<Sample>
- 2
<Protocol>
See Protocol 2
(temperature in annealing)
- 2:70.0℃
2:Digestion(1)
<Member>
Mariko,mizuki
<Sample>
- 1H:Avr2,FD Buffer
- 2(9/6):Nhe1、M Buffer
- 4(8/30):Avr2,FD Buffer
- 5(8/30):Spe1、M Buffer
<Protocol>
See Prptpcpl 9
3:Electrophoresis
<Member>
Mariko,mio
<Sample>
- PCR products(9/11)
- Digestion products(9/11)
<Protocol>
See Protocol 8
<Result>
.JPG)
4:Electrophoresis
<Member>
mio
<Sample>
Digestion products (9/11)
- 1
- 2
- 4
- 5
<Protocol>
See Protocol 8
<Result>
5:DNA extraction
<Member>
mizuki,easily
<Sample>
PCR products(9/11)
<Protocol>
See Protocol 4
6:Digestion(2)
<Member>
easily
<Sample>
- 1(9/11)
<Protocol>
See Prptpcpl 9
7:Electrophoresis
<Member>
Mariko,mizuki
<Sample>
Digestion products
- 1(9/11)
- 4L(8/26)
- 8H
<Protocol>
See Protocol 8
<Result>
.JPG)
8:DNA extraction
<Member>
mio,mizuki
<Sample>
- 4(9/11)
- 5(9/11)
<Protocol>
See Protocol 4
9:Ligation
<Member>
Mariko,mio
<Sample>
- 4(9/11)
- 5(9/11)
<Protocol>
See Protocol 10
10:DNA extraction
<Member>
Mariko,mio,mizuki
<Sample>
- 1(9/11)
- 2(9/11)
<Protocol>
See Protocol 4
12:Ligation
<Member>
Mariko
<Sample>
- 1(9/11)
- 2(9/11)
<Protocol>
See Protocol 10
