Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/17

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(New page: ===1:PCR=== <Member><br /> mio <Sample><br />(temperature in annealing) *3 *6 *8 <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Ph...)
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Revision as of 05:56, 28 September 2010

Contents

1:PCR

<Member>
mio


<Sample>
(temperature in annealing)

  • 3
  • 6
  • 8


<Protocol>
See Protocol 1



2：DNA Digestion

＜Member＞
mizuki


＜Sample, Materials＞
・PCR productions

  • 1 (8/31)
  • 2 (9/6)
  • 4 (8/31)
  • 5L (8/26)
  • 7 (8/31)
  • 8 (8/31)

・Digest enzyme

  • 1,4,8 : AvrⅡ
  • 2,7 : NheⅠ
  • 5L : SpeⅠ


＜Protocol＞
See Prptpcpl 9


3:Electrophoresis①

<Member>
mio


<Sample>
PCR products (9/17)

  • 3
  • 6
  • 8


<Protocol>
See Protocol 8


<Result>


4:Electrophoresis②

<Member>
mizuki


<Sample>
Digestion products (9/17)

  • 1
  • 2
  • 4
  • 5
  • 7
  • 8


<Protocol>
See Protocol 8


<Result>


5：DNA extraction from gels

＜Member＞
mizuki


＜Sample＞
Digestion products(9/17)

  • 4
  • 7
  • 8

(After electrophoresis)


＜Protocol＞
See Protocol 4


6：DNA Ligation

＜Member＞
mizuki


＜Sample＞

  • 7(9/17)
  • 8(9/17)

(after digestion)


＜Protocol＞
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/17"