"

From 2010.igem.org

Revision as of 00:55, 28 September 2010 (view source) Sandock (Talk | contribs) ← Older edit		Revision as of 01:03, 28 September 2010 (view source) Sandock (Talk | contribs) Newer edit →
Line 15:		Line 15:
	</div>		</div>
	</html>		</html>
		+
		+	Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:
		+	* Genebank file will all features (.gbk???)
		+	* Fwd and Reverse sequencing files (.ab1)
		+	* Gels from cloning and confirmation (.pdf)
		+	* All associated testing files and Results (.xls, .pdf, etc.)
		+
		+
	==List of Parts==		==List of Parts==
Line 23:		Line 31:
	|'''Function'''		|'''Function'''
	|'''Expression Type'''		|'''Expression Type'''
		+	|'''Zip File'''
	|-		|-
	|<partinfo>BBa_k318500</partinfo>		|<partinfo>BBa_k318500</partinfo>
	|Produces Trascription Factor RcsB		|Produces Trascription Factor RcsB
	|Inducible - IPTG		|Inducible - IPTG
		+	|500
	|-		|-
	|<partinfo>BBa_k318501</partinfo>		|<partinfo>BBa_k318501</partinfo>
	|Produces Trascription Factor RcsA		|Produces Trascription Factor RcsA
	|Inducible - IPTG		|Inducible - IPTG
		+	|501
	|-		|-
	|<partinfo>BBa_k318502</partinfo>		|<partinfo>BBa_k318502</partinfo>
	|Produces Trascription Factor RcsA & RcsB		|Produces Trascription Factor RcsA & RcsB
	|Inducible - IPTG		|Inducible - IPTG
		+	|502
	|}		|}

---

Revision as of 01:03, 28 September 2010

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

• Genebank file will all features (.gbk???)
• Fwd and Reverse sequencing files (.ab1)
• Gels from cloning and confirmation (.pdf)
• All associated testing files and Results (.xls, .pdf, etc.)

List of Parts

Enzyme Delivery Testing Constructs

Part Number	Function	Expression Type	Zip File
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factor RcsB	Inducible - IPTG	500
<partinfo>BBa_k318501</partinfo>	Produces Trascription Factor RcsA	Inducible - IPTG	501
<partinfo>BBa_k318502</partinfo>	Produces Trascription Factor RcsA & RcsB	Inducible - IPTG	502

Enzyme Delivery Final Constructs

Part Number	Function	Expression Type
<partinfo>BBa_k318500</partinfo>	Produces Trascription Factors RcsA & RcsB	Constitutive
<partinfo>BBa_k318500</partinfo>	Produces Beta-Galactasidase	Constitutive
<partinfo>BBa_k318500</partinfo>	A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA)	pH 2
<partinfo>BBa_k318500</partinfo>	A) Activates Lysis once in the small intestine	(+)-LuxR, (-)-cILVA
<partinfo>BBa_k318500</partinfo>	B) Activates lysis once in the small intestine	Bile Salts

Team Favorites

The Key Plasmid

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318000 BBa_K318000]

Other Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]

Characterization

1. Part 1
2. Part 2

Part Files

Put your .zip files here:

(Remember to include a Genbank file, part description, gel images (if available), sequencing information, and any characterization information)