If only the nucleotide sequence were screened, it would have been easy for ill-intentioned people to bypass the software: modifying the DNA sequence such as the sequence doesn't mime the dangerous one, but in having the same resulting amino-acid chain. Changing each triplet of nucleotides by a different one but coding for the same amino-acid is enough to obtain such a result.

To test if our software, taking amino-acid into account, respond well to such a modification (codon optimization), we created 20 nucleotide sequence by randomly back-translated a select agent amino-acid sequence.

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